Saturday, May 11, 2019 1:45 pm – 2:30 pm Exhibitor Workshop Room 1
BioLegend
Presenter:
- Miguel A. Tam, Senior Manager, Product Realization and Marketing
Recent advances in single cell analysis and instruments, in parallel with next-generation sequencing, have enabled the generation of transcriptomic data in a high-throughput fashion, propelling the field of single cell RNA sequencing (scRNA-seq). However, despite the analytical power of scRNA-seq, important hallmarks of immune cell states, phenotype, and other cellular processes that heavily rely on protein expression are often not detected in scRNA-seq. In this workshop, we will describe recent developments in the use of TotalSeq™ oligo-antibody conjugates as these reagents integrate seamlessly into workflows that combine scRNA-seq and protein analysis, such as CITE-seq and REAP-seq. We will illustrate how some flow cytometry experimental complexity can be simplified with the use of these reagents, reducing the number of populations that need to be sorted before single cell analysis. In addition, we will show the use of cell hashing reagents, which enable sample multiplexing, resulting in reduced variability, lower cost, and optimization of single cell instrument and reagents use. Hashing antibodies can also be applied to nuclei, not just single cell suspensions, for cells that are difficult to isolate. With the development of the TotalSeq™ reagent suite, we hope to facilitate the next wave of legendary discovery in biomedical research.
